Major autohemotherapy (mAHT) is the most common form of ozone therapy. Typically, 100 to 120ml of a patient’s blood is drawn together with 10ml sodium citrate into a 250ml vacuum bottle. Depending on the type of ozone generator, 150ml of O2/O3 gas is injected into the vacuum bottle, either via a direct connection or via a syringe. After careful mixing, the O3-enriched blood is readministered into the patient.
For therapies requiring higher dosing, the same procedure can also be performed using a 500ml vacuum bottle or with multi-pass protocols.